GSM5721016: ChIP-Seq GATA Splice-rep2 [G2021 113 12]; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
GATA3
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
T47D
cell line
T47D
cell type
breast cancer
cell subtype
luminal
genotype/variation
Splice del expressed
passages
<10
antibody
GATA3 (Cell Signaling, D13C9)
Sequenced DNA Library
library_name
GSM5721016
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNAs from various cells were purified by the Direct-zol RNA kit (Zymo). RNA-seq libraries were prepared using NEBNext Ultra II RNA-Seq Kit (NEB) as per the manufacturer's instruction with duel indexing. Quality of libraries was assessed in Tapestation (Agilent technologies) using D1000 Tape. Libraries were quantified using a well plate reader (Bio Tek) and pooled at a concentration of 12nM. Pooled libraries were quantified using the Droplet PCR system (Bio-Rad), and pooled libraries were sequenced in one lane Nova-Seq S4 as 150 bp Paired-end reads.